Fig. 2

a Time courses of biomass, glucose, iBuOH, acetate, and ethanol concentrations of strains SB001-pIBA and RL3000Δferm-pIBA4 cultivated anaerobically in minimal medium with (+ Ace) and without (−Ace) acetate supplementation. The means (solid lines) and individual data (dots) of n = 3 biologically independent samples are shown. b Carbon balance of biomass and quantified metabolites consumed (in) and produced (out) by strain SB001-pIBA in minimal medium with acetate supplementation. A molar ratio of 2 mol_CO2 mol_iBuOH⁻¹ were assumed to calculate the amount of produced CO₂ and a biomass sum formula of C_1.00H_1.77O_0.49N_0.24 to calculate the amount of C-atoms contained in the produced biomass. The amount of biomass and metabolites were normalized to the number of C-atoms they are composed of. The means (solid columns) and individual data (dots) of n = 3 biologically independent samples are shown. The error bars represent s.d