Fig. 3

a Time courses of biomass, glucose, iBuOH, acetate, and ethanol concentrations in the medium (left) and accumulated acetate and glucose consumption as well as estimated total production of ethanol and iBuOH (right) of strain SB001-pIBA in pulsed fed-batch bioreactor cultivation. The theoretical iBuOH and ethanol product titers were calculated from the total consumption of acetate and glucose with the experimentally determined yields of ethanol/acetate (0.96 mol mol⁻¹) and iBuOH/glucose (0.89 mol mol⁻¹) (see Table 1). b Time courses of specific glucose uptake rate and estimated volumetric iBuOH productivity of strain SB001-pIBA in pulsed fed-batch bioreactor cultivation. A corresponding replicate of the cultivation is shown in Additional file 1: Fig. S1