Table 1 Growth rates, substrate uptake rates, product excretion rates, ATP maintenance rate, product yields, and volumetric iBuOH productivity for strains SB001-pIBA4 and RL3000Δferm-pIBA4 cultivated anaerobically in minimal medium with (+ Ace) and without (−Ace) acetate

µ

[h⁻¹]

0.051 ± 0.001

0.051 (fixed)

 ~ 0

 ~ 0

 ~ 0

r_{Glc_up}

[mmol gDW⁻¹ h⁻¹]

4.80 ± 0.06

4.80 (fixed)

2.46 ± 1.33

1.15 ± 0.28

2.42 ± 0.99

r_{Iso_ex}

[mmol gDW⁻¹ h⁻¹]

4.36 ± 0.04

4.36 (fixed)

1.60 ± 0.16

1.14 ± 0.02

1.61 ± 0.08

r_{Ace_up}

[mmol gDW⁻¹ h⁻¹]

1.18 ± 0.10

1.18 (fixed)

 ~ 0

 ~ 0

 ~ 0

r_{Eth_ex}

[mmol gDW⁻¹ h⁻¹]

1.15 ± 0.02

0.93 (calculated)

 ~ 0

 ~ 0

 ~ 0

r_{CO2_ex}

[mmol gDW⁻¹ h⁻¹]

n.d

9.77 (calculated)

n.d

n.d

n.d

r_ATPM

[mmol gDW⁻¹ h⁻¹]

n.d

4.05 (calculated)

n.d

n.d

n.d

Y_Iso/Glc

[mol mol⁻¹]

0.89 ± 0.02

0.91 (fixed by given rates)

0.60 ± 0.16

0.67 ± 0.21

0.70 ± 0.38

Y_Eth/Ace

[mol mol⁻¹]

0.96 ± 0.05

0.79 (calculated)

n.d

n.d

n.d

Y_BM/Glc

[gDW g⁻¹]

0.057 ± 0.001

0.059 (calculated)

 ~ 0

 ~ 0

 ~ 0

q_Iso (2–48 h)

[mmol L⁻¹ h⁻¹]

1.33 ± 0.03

(n.d. by FBA)

0.14 ± 0.00

0.08 ± 0.00

0.15 ± 0.01