Fig. 2From: Construction of a plasmid-free l-leucine overproducing Escherichia coli strain through reprogramming of the metabolic fluxOptimization of the pathway for l-leucine biosynthesis and cell growth. A Introduction of 2-isopropylmalate synthase (IPMS) encoding genes from Corynebacterium glutamicum and Escherichia coli; B overexpression of the leuAfbr-ecj gene in the chromosome; C overexpression of l-leucine operon genes; D removal of the transcriptional attenuation of leuABack to article page