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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Construction of a plasmid-free l-leucine overproducing Escherichia coli strain through reprogramming of the metabolic flux

Fig. 2

Optimization of the pathway for l-leucine biosynthesis and cell growth. A Introduction of 2-isopropylmalate synthase (IPMS) encoding genes from Corynebacterium glutamicum and Escherichia coli; B overexpression of the leuAfbr-ecj gene in the chromosome; C overexpression of l-leucine operon genes; D removal of the transcriptional attenuation of leuA

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