Table 1 Escherichia coli strains used and constructed in this study
Strains | Characteristics | Source |
|---|---|---|
JM109 | Cloning host | This laboratory |
W3110 | Wild type, starting strain | This laboratory |
LEU01 | W3110, pTrc99a-leuAfbr-cgb | This study |
LEU02 | W3110, pTrc99a-leuAfbr-ecj | This study |
LEU03 | W3110, ΔlacI, ycjV::Ptrc-leuAfbr-ecj | This study |
LEU04 | LEU03, yciQ::Ptrc-leuAfbr-ecj | This study |
LEU05 | LEU04, yghE::Ptrc-leuAfbr-ecj | This study |
LEU06 | LEU05, yjgX::Ptrc-leuBCD | This study |
LEU07 | LEU06, yjiT::Ptrc-ilvIHfbr | This study |
LEU08 | LEU07, ylbE::Ptrc-ilvIHfbr | This study |
LEU09 | LEU08, yjiV::Ptrc-ilvEDC | This study |
LEU10 | LEU09, PleuA::Ptrc | This study |
LEU11 | LEU10, ΔlivK | This study |
LEU12 | LEU11, ΔlivJ | This study |
LEU13 | LEU11, livJ::Ptrc-yeaS | This study |
LEU14 | LEU11, livJ::Ptrc-azlC | This study |
LEU15 | LEU11, livJ::Ptrc-brnFE | This study |
LEU16 | LEU14, brnQ::Ptrc-brnFE | This study |
LEU17 | LEU16, yjiP::pntAB | This study |
LEU18 | LEU17, gltB::Ptrc-rocG | This study |
LEU19 | LEU17, ilvE::Ptrc-Esldh | This study |
LEU20 | LEU17, ilvE::Ptrc-Bcldh | This study |
LEU21 | LEU20, ΔpoxB | This study |
LEU22 | LEU21, ΔpflB | This study |
LEU23 | LEU22, ΔldhA | This study |
LEU24 | LEU23, yeeP::PapFAB104-esaR | This study |
LEU25 | LEU24, PsucAB::PesaS | This study |
LEU26 | LEU25, trpR::Pbs1-esaI | This study |
LEU27 | LEU25, trpR::Pbs2-esaI | This study |
LEU28 | LEU25, trpR::Pbs3-esaI | This study |
LEU29 | LEU25, trpR::Pbs4-esaI | This study |
LEU30 | LEU25, trpR::Pbs5-esaI | This study |