Fig. 3

Effects of overexpressing bldD on transcription levels of ilamycins biosynthesis gene cluster at 48 h and 72 h. A Ilamycins biosynthesis gene cluster and predicted promoters P1–P6. B Transcription level changes of intra-cluster genes in ΔR::bldD compared to ΔR. (Two internal reference genes hrdB and 08190 were used). a 48 h, hrdB as internal reference gene; b 48 h, 08190 as internal reference gene; c 72 h, hrdB as internal reference gene; d 72 h, 08190 as internal reference gene; e fold changes of transcription levels of ilamycins biosynthesis gene cluster in strains ΔR and ΔR::bldD within 48 to 72 h. Error bars represent the standard deviations from three independent biological replicates