Table 1 Bacterial strains and plasmids used or constructed in this work
Strains and plasmids | Relevant phenotype | Source |
|---|---|---|
Strains | ||
E. coli | ||
DH5α | F-ϕ80lacZΔM15Δ(lacZYA-argF)U169recA1endA1hsdR17(rk-mk +)phoAsupE44λ-thi-1gyrA96relA1 | From Prof Wu |
S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | From Prof Wu |
S. atratus | ||
SCSIO ZH16 | Wild type strain, ilamycins producer | From Prof Ma |
ΔR | SCSIO ZH16 with in-frame deletion of ilaR | Lab stock |
ΔR::ilaJ | ΔR Φ31 attB::pIBilaJ | This study |
ΔR::ilaK | ΔR Φ31 attB::pIBilaK | This study |
ΔR::ilaJ-ilaK | ΔR Φ31 attB::pIBilaJK | This study |
ΔR::bla | ΔR Φ31 attB::pIBbla | This study |
ΔR::PPtase1 | ΔR Φ31 attB::pIBPPtase1 | This study |
ΔR::PPtase2 | ΔR Φ31 attB::pIBPPtase2 | This study |
ΔR::PPtase3 | ΔR Φ31 attB::pIBPPtase3 | This study |
ΔR::sfp-svp | ΔR Φ31 attB::pWHU2449 | This study |
ΔR::shbA | ΔR Φ31 attB::pIBshbA | This study |
ΔR::bldD | ΔR Φ31 attB::pIBbldD | This study |
ΔR::arpA | ΔR Φ31 attB::pIBarpA | This study |
ΔR::afsR | ΔR Φ31 attB::pIBafsR | This study |
ΔR::rok7B7 | ΔR Φ31 attB::pIBrok7B7 | This study |
ΔR::bldA | ΔR Φ31 attB::pIBbldA | This study |
ΔR::adpA | ΔR Φ31 attB::pIBadpA | This study |
ΔR::sigF | ΔR Φ31 attB::pIBsigF | This study |
ΔR::sigN | ΔR Φ31 attB::pIBsigN | This study |
ΔR::ilaQ | ΔR Φ31 attB::pIBilaQ | This study |
ΔR::CS | ΔR Φ31 attB::pIBCS | This study |
ΔR::SKD | ΔR Φ31 attB::pIBSKD | This study |
ΔRΔatra | ΔR with in-frame deletion of atr23(RS26165) in BGC13 | This study |
Plasmids | ||
pKCcas9dO | A CRISPR/Cas9 editing plasmid harboring an actII-ORF4-specific gRNA and two homologous arms for in-frame deletion, aac(3)IV, pSG5 | [1] |
pKCcas9datr23 | A CRISPR/Cas9 editing plasmid harboring an atr23(RS26165)-specific gRNA and two homologous arms for in-frame deletion, aac(3)IV, pSG5 | This study |
pIB139 | Integrative vector based on Φ31 int/attP, aac(3)IV, oriT RK2, PermE* | [2] |
pIBilaJ | pIB139 harboring ilaJ under control of PermE* | This study |
pIBilaK | pIB139 harboring ilaK under control of PermE* | This study |
pIBilaJK | pIB139 harboring ilaJ-ilaK under control of PermE* | This study |
pIBbla | pIB139 harboring RS37870 under control of PermE* | This study |
pIBPPtase1 | pIB139 harboring RS01025 under control of PermE* | This study |
pIBPPtase2 | pIB139 harboring RS31655 under control of PermE* | This study |
pIBPPtase3 | pIB139 harboring RS38670 under control of PermE* | This study |
pWHU2449 | pIB139 harboring sfp-svp under control of PermE* | [3] |
pIBshbA | pIB139 harboring RS19455 under control of PermE* | This study |
pIBbldD | pIB139 harboring RS07905 under control of PermE* | This study |
pIBarpA | pIB139 harboring RS12555 under control of PermE* | This study |
pIBafsR | pIB139 harboring RS24115 under control of PermE* | This study |
pIBacsR | pIB139 harboring RS33465 under control of PermE* | This study |
pIBrok7B7 | pIB139 harboring RS32300 under control of PermE* | This study |
pIBbldA | pIB139 harboring RS16910 under control of PermE* | This study |
pIBadpA | pIB139 harboring RS15155 under control of PermE* | This study |
pIBsigF | pIB139 harboring RS21525 under control of PermE* | This study |
pIBsigN | pIB139 harboring RS21520 under control of PermE* | This study |
pIBilaQ | pIB139 harboring ilaQ under control of PermE* | This study |
pIBCS | pIB139 harboring RS07940 under control of PermE* | This study |
pIBSKD | pIB139 harboring RS07945 under control of PermE* | This study |