Fig. 2

Endolysin LysMP inhibition of susceptible bacteria. A Dose-dependent growth inhibition of purified LysMP against L. fermentum 0315-25 over time. B Antimicrobial spot assay of LysMP on polyacrylamide gel with target bacterium. Sterile 1 × PBS served as negative control (-Ctrl) and purified endolysin LysMP as treatment (LysMP). C Bacterial viability assay. Target bacterium with and without 30 min LysMP treatment (100 µg/mL). Bright-field images detect all cells. Green, fluorescent signal detect dead bacteria with compromised cell membrane. Red cells detect live bacterial cells