Fig. 4
Biochemical characterization of LysMP. A Calcium ion (CaCl2) at 0.1 mM and 1 mM were added to endolysin LysMP treatment. B Copper ion (CuCl2) at 0.1 mM and 1 mM were added to endolysin LysMP treatment and OD600 was used to determined growth of target bacteria over a period of 9 h at 37℃. C Iron(II) (FeCl2) at 0.1 mM and 1 mM were added to endolysin LysMP treatment. D Magnesium iron (MgCl2) at 0.1 mM and 1 mM were added to endolysin LysMP treatment. E Zinc iron (ZnCl2) at 0.1 mM and 1 mM were added to endolysin LysMP treatment. The OD600 was used to determined growth of target bacteria L. fermentum 0315-25 over a period of 9 h at 37 ℃. Data are means ± standard deviations (n = 3) using analysis of variant. *p < 0.05; **p < 0.01; ****p < 0.00001