Fig. 8

SDS–PAGE analysis. Lane 1 in (A–C, E–G) is loaded with BioRad pre-stained protein ruler (10–250 kDa) (only the part of the gel showing proteins around 36 kDa is shown here). Letters C, F and A denote proteins OmpC, OmpF and OmpA, respectively. A E. coli Bw25113 grown under low osmolarity (lane 2), E. coli Bw25113 (lane 3), ΔompC (lane 4) ΔompF (lane 5) and ΔompA (lane 6) grown in LB Broth with 5 g/L glucose. *2 denotes lane 2 with the positions of the protein bands marked separately due to space constraints. B E. coli Bw25113 grown in LB Broth (with 5 g/L glucose) (lane 1), E. coli Bw25113 grown in LB Broth (with 5 g/L glucose) in the presence of ethanol (lane 2), propanol (lane 3), butanol (lane 4), pentanol (lane 5) and hexanol (lane 6) at concentrations leading to ~ 50% growth inhibition. C Two biological replicates of E. coli Bw25113 grown in LB Broth with 5 g/L glucose and 5 mM EDTA (lanes 3 and 4). D Concentration of butanol leading to ~ 50% growth inhibition (MIC₅₀) in different strains of E. coli grown in LB Broth (with 5 g/L glucose). Two biological replicates of E. coli (E) W3110, F WBB06 and (G) K12 (ATCC 10798) grown in the presence (lanes 5 and 6) and absence (lanes 3 and 4) of butanol. LB Broth (with 5 g/L glucose) was used as the growth media for these strains and butanol was added at concentrations leading to 50% growth inhibition, as shown in (D)