Fig. 2

Heterotrophic ectoine and PHB production of H. rowanensis. A Halomonas metabolic pathway to ectoine and PHB production. Gene annotations from H. rowanensis are shown in blue. B Ectoine production of H. rowanensis (green) and H. bluephagenesis TD1.0 (blue) in variable levels of salinity. Cultures were harvested and milked for ectoine with ultrapure water as described previously [35]. C PHB production of H. rowanensis and H. bluephagenesis TD1.0 in LB60 with and without 4% glucose. Cultures were grown for 24 h and the PHA concentration was determined by polymer extraction, degradation, derivatization and GC quantitation. Error bars represent one standard deviation of triplicate analyses. Enzymes: asd = aspartate semialdehyde dehydrogenase 1; EctA = L-2,4-diaminobutyric acid acetyltransferase; ectB = L-2,4-diaminobutyric acid transaminase; ectC = L-ectoine synthase; ectD = ectoine dioxygenase; lysC = aspartate kinase; phaA = 3-ketothiolase; phaB = NADPH-dependent acetoacetyl-CoA reductase; phaC = PHA synthase. The PHB titre and CDW data are available in Additional file 1: Table S2