Fig. 3

Chemoautotrophic growth of H. rowanensis. A Chemoautotrophic growth of three Halomonas species. Cultures were grown in thiosulfate growth medium containing 150 mM NaHCO₃ at 30 °C for 48 h. B Growth of H. rowanensis with or without supplemental inorganic carbon. Cultures were grown in thiosulfate medium in the presence and absence of 150 mM NaHCO₃ for 100 h at 30 °C. C Radiolabelled carbon fixation of non-permeabilised cultures. Cultures were grown in species-specific growth medium and the ¹⁴C (H¹⁴CO₃⁻) fixation assay was performed as described previously [37] with the culture at OD 600 nm = 4.0. D Time course of ¹⁴C labelled bicarbonate fixation in six microorganisms. Cells (OD 600 nm = 4.0) were permeabilised with alkyltrimethylammonium bromide with NaH¹⁴CO₃ addition to allow the measurement of total enzymatic carbon fixation activity independently of bicarbonate uptake systems. The H. bluephagenesis strain for parts C, D was TD1.0