Fig. 6
Propane production of H. bluephagenesis TQ10 and/or H. rowanensis expressing plasmid borne CvFAPG462V with a (A) weak p7 and (B) strong p102 constitutive promoter. In part A, cultures (50 mL) were cultivated in LB60 medium (H. bluephagenesis TD1.0) or thiosulfate medium (H. rowanensis) for 6 h from a 1% inoculum at 30 °C and 200 rpm. Triplicate aliquots (1 mL) were sealed in 4 mL glass vials and incubated overnight at 30 °C and 180 rpm under a blue LED panel. For part B, H. rowanensis was cultivated as above in the two medium types with or without supplemental butyric acid (10 mM). Propane was quantified by Micro GC analysis of manual headspace samples. C Ectoine production of H. rowanensis in different growth media all with 10% (w/v) NaCl except the brine water which is ~ 20% NaCl. Cells were harvested by centrifugation and milked to release ectoine after 48 h in shake flasks at 30 °C. Ectoine and hydroxyectoine were quantified by HPLC. D Box plot of PHA production. PHB was accumulated over 48 h in LB60 and thiosulfate media in shake flasks at 30 °C. Cells from 50 mL were harvested by centrifugation and freeze dried. CDW was recorded and PHB concentration was determined by GC after derivatisation. Data are shown as the g PHB per g of freeze dried CDW starting material. HR = H. rowanensis; TD01 = H. bluephagenesis TD01 (wild-type isolate). The PHB titre and CDW data are available in Additional file 1: Table S2