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Table 1 Chemical composition of washed pretreated solids prior to enzymatic saccharification at 12.5% WIS with varied enzyme preparation loadings and after 72 h reactions with air or N2a,b

From: LPMO-supported saccharification of biomass: effects of continuous aeration of reaction mixtures with variable fractions of water-insoluble solids and cellulolytic enzymes

Constituent

PS

PS-Air

PS-N2

6%

9%

12%

6%

9%

12%

Glucan

48.3 (1.9)

31.9 (0.3)

26.6 (0.4)

26.9 (0.3)

41.0 (0.1)

39.2 (0.2)

36.8 (0.1)

Xylan

 ≤ 0.1

 ≤ 0.1

 ≤ 0.1

 ≤ 0.1

 ≤ 0.1

 ≤ 0.1

 ≤ 0.1

Mannan

 ≤ 0.3

 ≤ 0.3

 ≤ 0.3

 ≤ 0.3

 ≤ 0.3

 ≤ 0.3

 ≤ 0.3

Arabinan

ND

ND

ND

ND

ND

ND

ND

Galactan

ND

ND

ND

ND

ND

ND

ND

Klason lignin

42.1 (2.2)

54.2 (3.0)

56.4 (2.2)

55.4 (0.7)

46.3 (4.0)

46.7 (1.5)

49.6 (2.3)

ASL

6.6 (0.4)

5.8 (0.3)

5.9 (0.3)

6.1 (0.5)

5.7 (0.2)

5.8 (0.1)

5.4 (0.4)

  1. aDetermined using two-step treatment with sulfuric acid (TSSA). Values given as mass fraction in percent dry weight with standard deviation in parentheses. The values are averages of six measurements (duplicate samples of solid fractions and triplicate analyses of each sample)
  2. bASL acid-soluble lignin, PS pretreated solids, ND not detected, WIS water-insoluble solids