Skip to main content

Table 2 Glucose yields from enzymatic saccharification of reaction mixtures with varied WIS and varied enzyme preparation loadingsa,b

From: LPMO-supported saccharification of biomass: effects of continuous aeration of reaction mixtures with variable fractions of water-insoluble solids and cellulolytic enzymes

Reaction conditions

Produced glucose (g/L)c,d

24 h

48 h

72 h

WIS contenta

 12.5%

Air

28.6 (2.7) ***

31.9 (1.0) ***

34.6 (2.2) ***

 12.5%

N2

13.5 (1.7)

17.9 (1.9)

18.9 (0.7)

 15.0%

Air

26.1 (2.2) ***

34.8 (2.3) ***

38.8 (1.8) ***

 15.0%

N2

15.1 (3.2)

19.5 (2.6)

24.5 (1.9)

 17.5%

Air

22.7 (4.0) ***

32.5 (0.7) ***

39.7 (2.2) ***

 17.5%

N2

16.9 (0.7)

17.5 (1.9)

22.1 (3.1)

Enzyme preparationb

 6%

Air

25.3 (3.4) ***

33.1 (4.1) ***

37.1 (4.4) ***

 6%

N2

15.0 (1.5)

18.3 (1.9)

19.9 (1.1)

 9%

Air

32.3 (0.9) ***

37.9 (2.9) ***

43.6 (1.9) ***

 9%

N2

19.3 (2.0)

21.8 (2.8)

25.9 (1.8)

 12%

Air

37.0 (1.6) ***

44.2 (0.5) ***

47.3 (1.8) ***

 12%

N2

19.7 (1.9)

22.0 (3.5)

25.9 (2.4)

  1. aThe series with varied content of water-insoluble solids (WIS) was performed with 6% enzyme preparation
  2. bThe series with varied enzyme loadings was performed with 12.5% WIS
  3. cThe table shows the glucose that was produced during enzymatic saccharification reactions, i.e., the glucose that was in the slurry as a result of the pretreatment has been deducted
  4. dThe values shown are averages of nine measurements (triplicate reactions and triplicate analyses of each reaction). Standard deviations in parentheses
  5. Asterisks indicate significant differences (Student's t- test) between reactions with air and N2: ***p ≤ 0.01; ** 0.01 < p ≤ 0.05; * 0.05 < p ≤ 0.1