Biotechnology for Biofuels and Bioproducts

Table 3 Analysis of crystallinity of washed pretreated solids prior to enzymatic saccharification at 12.5% WIS with varied enzyme preparation loadings and after 72 h enzymatic reaction with air or N₂^a

From: LPMO-supported saccharification of biomass: effects of continuous aeration of reaction mixtures with variable fractions of water-insoluble solids and cellulolytic enzymes

	PS	PS-Air			PS-N₂
	PS	6%	9%	12%	6%	9%	12%
XRD
CrI %	64.4	49.9	47.5	44.4	59.3	62.6	60.3
2θ I_AM (°)	18.92	19.18	19.12	18.85	18.96	18.99	18.73
I_AM (CPS)	2.42	4.56	2.99	8.18	2.99	2.48	7.83
2θ I₂₀₀ (°)	22.57	22.57	22.57	22.63	22.58	22.57	19.72
I₂₀₀ (CPS)	6.81	9.09	5.70	14.7	7.51	6.63	22.66
¹³C NMR^b
Int.(89)	1307	961	865	865	1172	1145	1114
Int.(84)	716	544	497	496	626	626	606
Int.(89)/Int.(84)	1.83	1.77	1.74	1.74	1.85	1.83	1.84
CrI (%)	64.6	63.9	63.5	63.6	64.9	64.7	64.8

^aCPS counts per second, CrI crystallinity index, PS pretreated solids, NMR nuclear magnetic resonance spectroscopy, WIS water-insoluble solids, XRD X-ray diffraction
^bThe relative intensities of the peaks at 89 ppm (crystallinity region) and 84 ppm (amorphous region) are listed

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ISSN: 2731-3654

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