Fig. 1

Loop analysis of the active site architecture of GH5_2 cellulases. a TfCel5A-cellopentose complex. The loops of the active site architecture are shown in colors. Loops 1, 2, 5, and 6 are shown in salmon; loops 3 and 4 are shown in blue; and loops 7 and 8 are shown in magenta and cyan, respectively. Cellopentose in the active cleft is shown in green. b Hydrogen bond network between the residues in loops 1–8 and the ligand. The subsites of the active cleft are labeled from − 3 to + 2. c Sequence profile of the active site architecture of GH5_2 enzymes. Based on the cut-off of 5 Å, the amino acid residues surrounding cellopentose in the TfCel5A-cellopentose complex were selected to generate the reference using PyMOL software. The size of the letter indicates the degree of conservation of the residues. The sugar atoms interacting with the enzyme are labeled above the residue