Fig. 5

Comparison of catalytic activities between the WT and its mutants at 37 °C, 50 °C, and 60 °C. a The amino acid positions selected for site-directed mutations. Loops 7 and 8 are shown in red and cyan, respectively. The subsites of the active site are labeled from − 3 to + 2. b The relative catalytic activity of TfCel5A mutants at different temperatures