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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: De novo biosynthesis of 2-hydroxyterephthalic acid, the monomer for high-performance hydroxyl modified PBO fiber, by enzymatic Kolbe–Schmitt reaction with CO2 fixation

Fig. 3

2-HTA production by whole-cell reaction of E. coli BL21(DE3) harboring different 2,3-DHBD. a Reaction conditions: 30 mg/mL lyophilized whole cells, 10 mM substrate, 3 M KHCO3, 30 ℃, 180 rpm. b Enzyme: 2,3-DHBD_Aoopt: 2,3-DHBD_Ao with codon-optimized; 2,3-DHBD_Foopt: 2,3-DHBD_Fo with codon-optimized; sequences of native and codon-optimized genes were shown in Additional file 1: Table S3. c One-way analysis of variance (one-way ANOVA) was used to analyze the data

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