Table 1 Strains and plasmids used in this study
Strain | Genotype | Source |
|---|---|---|
E. coli BW25113/F’ | Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 [F’ proAB lacIqZDM15Tn10 (TetR)] | Yale CGSC |
E. coli Trans5α | F-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (rk -, mk +) supE44λ- thi-1 gyrA96 relA1 phoA | Stratagene |
E. coli BL21(DE3) | F−ompT hsdSB (rB−mB−) gal dcm (DE3) | Invitrogen |
S01 | BW25113/F′ harboring pZE12-araABC and pCS-mdlC-yqhD | This study |
S02 | BW25113/F′ harboring pZE12-araABC and pCS-aro10-yqhD | This study |
S03 | BW25113/F′ harboring pZE12-araABC and pCS-kivD-yqhD | This study |
S04 | BW25113/F′ harboring pZE12-araABC and pCS-kivD(V461I)-yqhD | This study |
S05 | BW25113/F′ harboring pZE12-araABC and pCS-kivD(S286T)-yqhD | This study |
S06 | BW25113/F′ harboring pZE12-araABC and pCS-kivD(V461I/S286T)-yqhD | This study |
S07 | BW25113/F′ harboring pCS-araABC, pZE-kivD(V461I/S286T)-yqhD | This study |
S08 | BW25113/F′ harboring pCS-araABC-yqhD, pZE-kivD(V461I/S286T) | This study |
S09 | BW25113/F′ harboring pCS-araABC, pZE-kivD(V461I/S286T) and pSA-yqhD | This study |
Plasmid | Description | Source |
|---|---|---|
pZE12-luc | pLlacO1; luc; ColE1 ori; AmpR | [6] |
pCS27 | pLlacO1; p15A ori; KanR | [8] |
pSA74 | PLlacO1; pSC101 ori; CmR | [29] |
pETDuet-1 | two T7 promoters; two MCS; pBR322 ori; AmpR | Novagen |
pZE-araABC | pZE12 containing araA, araB and araC from Burkholderia multivorans | This study |
pCS-mdlC-yqhD | pCS27 containing mdlC from Pseudomonas putida ATCC12633 and yqhD from E. coli | This study |
pCS-aro10-yqhD | pCS27 containing aro10 from Saccharomyces cerevisiae and yqhD from E. coli | This study |
pCS-kivD-yqhD | pCS27 containing kivD from Lactococcus lactis and yqhD from E. coli | This study |
pCS-kivD(V451I)-yqhD | pCS27 containing Kivd mutant V451I and yqhD from E. coli | This study |
pCS-kivD(S286T)-yqhD | pCS27 containing Kivd mutant S286T and yqhD from E. coli | This study |
pCS-kivD(V451I/S286T)-yqhD | pCS27 containing Kivd mutant V451I/S286T and yqhD from E. coli | This study |
pCS-araABC | pCS27 containing araA, araB and araC from Burkholderia multivorans | This study |
pCS-araABC-yqhD | pCS27 containing araA, araB and araC from Burkholderia multivorans and yqhD from E. coli | This study |
pZE-kivD(V451I/S286T) | pZE12 containing Kivd mutant V451I/S286T | This study |
pZE-kivD(V451I/S286T)-yqhD | pZE12 containing Kivd mutant V451I/S286T and yqhD from E. coli | This study |
pSA-yqhD | pSA74 containing yqhD from E. coli | This study |