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Table 2 Kinetic parameters of Kivd and its mutants toward 2-keto-3-deoxy-arabonate

From: Rational protein engineering of a ketoacids decarboxylase for efficient production of 1,2,4-butanetriol from arabinose

Enzyme

Substrate

Km (mM)

kcat (s−1)

kcat/Km (mM−1 s−1)

KivD

2-Keto-3-deoxy-arabonate

0.940 ± 0.127

0.396 ± 0.023

0.421

KivD V461I

2-Keto-3-deoxy-arabonate

0.572 ± 0.133

0.462 ± 0.036

0.807

KivD S286T

2-Keto-3-deoxy-arabonate

0.423 ± 0.070

0.458 ± 0.021

1.084

KivD V461I/S286T

2-Keto-3-deoxy-arabonate

0.278 ± 0.045

0.332 ± 0.012

1.189

  1. Enzyme activity was determined using a coupled enzyme assay. Data are presented as mean ± s.d. (n = 3)
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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