Table 1 Strains used in this study
From: Efficient production of mycosporine-like amino acids, natural sunscreens, in Yarrowia lipolytica
Strain | Genotype |
|---|---|
JHYG0 | Po1g ku70Δ ura3Δ |
JHYG1 | JHYG0 C3:: loxP-URA3-loxP |
JHYG2 | JHYG0 C3:: PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG3 | JHYG0 C3:: PTEF(406)-Np.mysD-TCYC1-PTEF(406)-Np.mysC-TCYC1-PTEF(406)-Np.mysB-TCYC1-PTEF(406)-Np.mysA-TCYC1-loxP-URA3-loxP |
JHYG4 | JHYG0 C3:: PTEF(406)-Np.mysD-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG5 | JHYG0 C3:: PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Np.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG6 | JHYG0 C3:: PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Np.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG7 | JHYG0 C3:: PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Np.mysA-TCYC1-loxP-URA3-loxP |
JHYG8 | JHYG0 C3:: PUAS1B8-TEF(136)-Np.mysD-TCYC1-PUAS1B8-TEF(136)-Av.mysC-TCYC1-PUAS1B8-TEF(136)-Av.mysB-TCYC1-PUAS1B8-TEF(136)-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG9 | JHYG0 C3:: PTEFin-Np.mysD-TCYC1-PTEFin-Av.mysC-TCYC1-PTEFin-Av.mysB-TCYC1-PTEFin-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG82 | JHYG8 E3:: PUAS1B8-TEF(136)-Np.mysD-TCYC1-PUAS1B8-TEF(136)-Av.mysC-TCYC1-PUAS1B8-TEF(136)-Av.mysB-TCYC1-PUAS1B8-TEF(136)-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG83 | JHYG82 D1:: PUAS1B8-TEF(136)-Np.mysD-TCYC1-PUAS1B8-TEF(136)-Av.mysC-TCYC1-PUAS1B8-TEF(136)-Av.mysB-TCYC1-PUAS1B8-TEF(136)-Av.mysA-TCYC1-loxP-URA3-loxP |
JHYG831 | JHYG83 AXP1:: PTEF(406)-Ps.XYL1-TCYC1-PTEF(406)-Ps.XYL2-TCYC1-PTEF(406)-Yl.XYL3-TCYC1-loxP-URA3-loxP |
JHYG832 | JHYG831 A1:: PUAS1B8-TEF(136)-GND1-TCYC1-lox71-URA3-lox66 |
JHYG833 | JHYG831 A1:: PUAS1B8-TEF(136)-ZWF1-TCYC1-lox71-URA3-lox66 |
JHYG834 | JHYG831 A1:: PUAS1B8-TEF(136)-GND1-TCYC1-PUAS1B8-TEF(136)-ZWF1-TCYC1-lox71-URA3-lox66 |
JHYG835 | JHYG831 pgi1Δ:: lox71-URA3-lox66 |
JHYGX1 | JHYG0 AXP1:: PTEF(406)-Yl.XYL1-TCYC1-PTEF(406)-Yl.XYL2-TCYC1-PTEF(406)-Yl.XYL3-TCYC1-loxP-URA3-loxP |
JHYGX2 | JHYG0 AXP1:: PTEF(406)-Ps.XYL1-TCYC1-PTEF(406)-Ps.XYL2-TCYC1-PTEF(406)-Yl.XYL3-TCYC1-loxP-URA3-loxP |