Table 2 Plasmids used in this study
From: Efficient production of mycosporine-like amino acids, natural sunscreens, in Yarrowia lipolytica
Plasmid | Description |
|---|---|
pYL-Cre-LEU2 | Cre recombinase expression plasmid containing PEXP1-Cre-TCYC1 and LEU2 marker |
Int C3 TEF AAAA | C3 site integration plasmid, PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
Int C3 TEF NNNN | C3 site integration plasmid, PTEF(406)-Np.mysD-TCYC1-PTEF(406)-Np.mysC-TCYC1-PTEF(406)-Np.mysB-TCYC1-PTEF(406)-Np.mysA-TCYC1-loxP-URA3-loxP |
Int C3 TEF AAAN | C3 site integration plasmid, PTEF(406)-Np.mysD-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
Int C3 TEF AANA | C3 site integration plasmid, PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Np.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
Int C3 TEF ANAA | C3 site integration plasmid, PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Np.mysB-TCYC1-PTEF(406)-Av.mysA-TCYC1-loxP-URA3-loxP |
Int C3 TEF NAAA | C3 site integration plasmid, PTEF(406)-Av.mysE-TCYC1-PTEF(406)-Av.mysC-TCYC1-PTEF(406)-Av.mysB-TCYC1-PTEF(406)-Np.mysA-TCYC1-loxP-URA3-loxP |
Int C3 UAS1B8 AAAN | C3 site integration plasmid, PUAS1B8-TEF(136)-Np.mysD-TCYC1-PUAS1B8-TEF(136)-Av.mysC-TCYC1-PUAS1B8-TEF(136)-Av.mysB-TCYC1-PUAS1B8-TEF(136)-Av.mysA-TCYC1-loxP-URA3-loxP |
Int C3 TEFin AAAN | C3 site integration plasmid, PTEFin-Np.mysD-TCYC1-PTEFin-Av.mysC-TCYC1-PTEFin-Av.mysB-TCYC1-PTEFin-Av.mysA-TCYC1-loxP-URA3-loxP |
Int E3 UAS1B8 AAAN | E3 site integration plasmid, PUAS1B8-TEF(136)-Np.mysD-TCYC1-PUAS1B8-TEF(136)-Av.mysC-TCYC1-PUAS1B8-TEF(136)-Av.mysB-TCYC1-PUAS1B8-TEF(136)-Av.mysA-TCYC1-loxP-URA3-loxP |
Int D1 UAS1B8 AAAN | D1 site integration plasmid, PUAS1B8-TEF(136)-Np.mysD-TCYC1-PUAS1B8-TEF(136)-Av.mysC-TCYC1-PUAS1B8-TEF(136)-Av.mysB-TCYC1-PUAS1B8-TEF(136)-Av.mysA-TCYC1-loxP-URA3-loxP |
Int AXP1 TEF Yl.XYL | AXP1 site integration plasmid, PTEF(406)-Yl.XYL1-TCYC1-PTEF(406)-Yl.XYL2-TCYC1-PTEF(406)-Yl.XYL3-TCYC1-loxP-URA3-loxP |
Int AXP1 TEF XYL | AXP1 site integration plasmid, PTEF(406)-Ps.XYL1-TCYC1-PTEF(406)-Ps.XYL2-TCYC1-PTEF(406)-Yl.XYL3-TCYC1-loxP-URA3-loxP |
Int A1 UAS1B8 GND1 | A1 site integration plasmid, PUAS1B8-TEF(136)-GND1-TCYC1-lox71-URA3-lox66 |
Int A1 UAS1B8 ZWF1 | A1 site integration plasmid, PUAS1B8-TEF(136)-ZWF1-TCYC1-lox71-URA3-lox66 |
Int A1 UAS1B8 GND1-ZWF1 | A1 site integration plasmid, PUAS1B8-TEF(136)-GND1-TCYC1-PUAS1B8-TEF(136)-ZWF1-TCYC1-lox71-URA3-lox66 |
Del PGI1 Δ | PGI1 deletion plasmid, PGI1 up 1 kb-lox71-URA3-lox66-PGI1 down 1 kb |