Fig. 2

XTR1 DBD binds to the xyn1 promoter. A–D Yeast one-hybrid analyses of binding ability of XTR1 DBD to the xyn1 or xyn2 promoter fragments including Pxyn1a (− 750 ~ − 91 bp) (A), Pxyn1b (− 1500 ~ − 750 bp) (B), Pxyn2b (− 1500 ~ − 750 bp) (C), and Pxyn2 (− 1500 ~ − 43 bp) (D). Serial dilutions of yeast transformant cells harboring the indicated plasmids were spotted on double dropout medium (-Leu/-Uracil) with or without AbA supplement, and were allowed to grow at 28 °C for 3 days. Yeast cells carrying pGADT7 with XTR1 DBD encoding sequence plus empty pAbAi or those with empty pGADT7 plus pAbAi harboring the indicated promoter fragments were set as negative controls