Fig. 5

XTR1 exerts a predominant regulatory effect on the transcription of xyn1. A, B Quantitative RT-PCR analyses of the relative transcription of xyn1 (A) and xyn2 (B) in QM9414, Δxtr1 and CpΔxtr1 cultivated on 0.5% xylan. C, D Western blot analyses of XYNI (C) and XYNII (D) production in the culture supernatant of QM9414 and Δxtr1 that were cultivated on xylan for the indicated time periods. E Western blot analyses of XYNI (upper) and XYNII (lower) production in the culture supernatant of QM9414, Δxtr1 and CpΔxtr1 cultivated on xylan for 60 h. Two sample repeats for each strain were shown. F Extracellular xylanase activity analyses of the culture supernatant from QM9414, Δxtr1 and CpΔxtr1 cultivated on xylan. Values are the mean of three biological replicates. Error bars are the SD from these replicates. Significant differences (t-test *P < 0.05, **P < 0.01, *** P < 0.001) were observed in the relative transcription level of xyn1 and xyn2 and extracellular xylanase activities between QM9414 and Δxtr1 or CpΔxtr1. Equal volume of culture supernatant (15 μL) of the relevant strains was applied for western blot analyses as shown in (C–E)