Fig. 9
From: A novel transcriptional repressor specifically regulates xylanase gene 1 in Trichoderma reesei
Determination of the critical nucleotides within xyn1 promoter for XTR1 binding via EMSAs. The Pxyn1 (− 321 ~ − 217 bp) fragments with the original or changed nucleotide sequence in the binding sites of XTR1 as shown were, respectively, incubated with the purified recombinant XTR1 DBD protein and subjected to EMSAs. The mutated nucleotides were indicated in red