Fig. 2

The effect of increasing intracellular Cys and Met content on biomass and CAZymes activities of NJAU4742. A The growth status of WT, OE-TgcysK, ∆TgcysK, OE-Tghmt, and ∆Tghmt on MM + straw and PDA, grown at 28 °C for 3 days, note that growth of strains on PDA was not different, but there was different on MM + straw. B Hyphae biomass of WT, OE-TgcysK, ∆TgcysK, OE-Tghmt and ∆Tghmt, strains were grown on MM + straw for at 28 °C 4 days. C FoldChange of TgcysK (52.35 folds up-regulation) in OE-TgcysK relative to WT and FoldChange of Tghmt (79.75 folds up-regulation) in OE-Tghmt relative to WT. D Intracellular Cys content of OE-TgcysK and intracellular Met content of OE-Tghmt, strains were grown on PDA at 28 °C 3 days. E FPA of WT, OE-TgcysK, ∆TgcysK, OE-Tghmt, and ∆Tghmt. Bars represent mean ± SEM, with n = 3 biological repeats; red dots resemble values from individual experiments. Student’s t-testing was conducted in (B, D, E), ***significant difference to WT at two-tailed P = 0.00011 (B, OE-TgcysK), 0.00017 (B, ∆TgcysK), 0.00047 (B, OE-Tghmt), ns = no statistical difference to WT at two-tailed P = 0.23 (B, ∆Tghmt); *significant difference to WT at two-tailed P = 0.020 (D, OE-Tghmt), no statistical difference to WT at two-tailed P = 0.051 (D, OE-TgcysK); ***significant difference to WT at two-tailed P = 0.00081 (E, OE-TgcysK), **significant difference to WT at two-tailed P = 0.0016 (E, ∆TgcysK), 0.0021 (E, OE-Tghmt), no statistical difference to WT at two-tailed P = 0.73 (E, ∆Tghmt)