Fig. 3

Transcriptome analysis of different strains including WT, OE-TgcysK, ∆TgcysK, OE-Tghmt, and ∆Tghmt. A PCA of transcriptomes (indicated by dots) in WT, OE-TgcysK, ∆TgcysK, OE-Tghmt, and ∆Tghmt. Hyphae were grown at 28 °C for 3 days on MM + straw. Clustering indicated similarity amongst data sets. The number of experiments: 2 (WT, OE-TgcysK, ∆TgcysK, OE-Tghmt and ∆Tghmt). B Correlation matrix analysis of transcriptomes of strains and their repeats. There was a great correlation between OE-Tghmt and ∆Tghmt (0.9942 and 0.9921). C Venn diagram showed the number of differentially expressed genes, significantly (P < 0.05) upregulated or downregulated in hyphae of strains OE-TgcysK, ∆TgcysK, OE-Tghmt, and ∆Tghmt, grown on MM + straw at 28 °C for 3 days. Data were obtained by comparison with WT, grown in the same condition. D Distribution of significantly (P < 0.05) up/down-regulated genes of OE-TgcysK relative to WT. E KEGG pathway classification on significantly (P < 0.05) upregulated genes of OE-TgcysK relative to WT, note that genes belonged to amino acid metabolism were up-regulated most, and other up-regulated genes were mainly distributed in energy, carbohydrate, and transport metabolism. F Gene ontology classification for significantly (P < 0.05) upregulated genes of OE-TgcysK relative to WT