Fig. 4

Effect of Tgmst1 on lignocellulolytic response and intracellular glucose content of NJAU4742. A The growth status of strains (WT, OE-Tgmst1, and ∆Tgmst1) on MM + straw, MM + glucose, and PDA, grown at 28 °C for 4 days, note that growth of strains on PDA has not obviously different, but there were different on MM + straw and MM + glucose. B Relative qPCR results of Tgmst1 in OE-Tgmst1, FoldChange was obtained by comparison with WT, Tgmst1 was 36.44 folds up-regulated in OE-Tgmst1. C FPA of strains (WT, OE-Tgmst1, and ∆Tgmst1) on MM + straw, grown at 28 °C 4 days, overexpression of Tgmst1 increased enzyme activity, while deletion leads to a decrease. D Hyphae biomass of strains (WT, OE-Tgmst1, and ∆Tgmst1), grown on MM + straw at 28 °C for 4 days. E The intracellular glucose content of strains (WT, OE-Tgmst1, and ∆Tgmst1), and hyphae were washed and ground, and glucose was extracted with buffer and determined. overexpression of Tgmst1 increased the intracellular glucose content. F Y2H assay indicated the strong interaction between MST1 and PKA, see Additional file 1: Fig. S6C for the original image. pGADT7-EV and pGBKT7-MST1 were transformed to verify the self-activate of MST1 in yeast, and the transformant cannot grow on SD-His-Leu-Trp; pGADT7-PKA and pGBKT7-MST1 were transformed to verify the interaction between MST1 and PKA, and the transformant can grow normally on SD-His-Leu-Trp. The yeast with a 10^–1 dilution ratio. SD (-Ade)-His-Leu-Trp is SD medium without (Adenine) Histidine, Leucine, and Tryptophan. Bars represent mean ± SEM, with n = 3 biological repeats; red dots resemble values from individual experiments. Student’s t-testing was conducted in (C, D, E), **significant difference to WT at two-tailed P = 0.0084 (C, OE-Tgmst1), 0.0043 (B, ∆Tgmst1); **significant difference to WT at two-tailed P = 0.0032 (C, OE-Tgmst1), ***significant difference to WT at two-tailed P = 0.00015 (C, ∆Tgmst1); *significant difference to WT at two-tailed P = 0.047 (E, OE-Tgmst1), 0.025 (E, ∆Tgmst1)