Fig. 5
The Optical glucose FRET sensor further indicated that MST1 facilitated the increase of intracellular glucose. A Growth status of TgGluSensor::CON and TgGluSensor::OE-Tgmst1 on MM + straw, grown at 28 °C for 4 days. B The newly grown hyphae of TgGluSensor::CON and TgGluSensor::OE-Tgmst1 were gently scraped by glass. FY and FC were observed by a CLSM. B1, 2 Fluorescence images of TgGluSensor::CON and TgGluSensor::OE-Tgmst1. YFP was represented by green, and CFP was represented by blue, which was helpful for differences observation. The top one was emitting light of YFP (green), the second one was emitting light of CFP (blue), the third was a merged image of YFP and CFP, and the bottom was a merged image of YFP and CFP in white light. YFP and CFP were excited at 500 nm and 430 nm respectively and received at 525 nm and 470 nm respectively; hyphae length in visual field was 60 µm; C Relative fluorescence intensity of hyphae was calculated by the software (ZEN3.4). D The intracellular glucose content of TgGluSensor::CON and TgGluSensor::OE-Tgmst1, which were grown at 28 °C for 4 days. E The mean value of FY/FC of TgGluSensor::CON (0.89) and TgGluSensor::OE-Tgmst1 (1.66) were obtained by counting pixel fluorescence intensity. Bars represent mean ± SEM, with n = 3 biological repeats; red dots resemble values from individual experiments. Student’s t-testing was conducted in (D, E), *significant difference to TgGluSensor::CON at two-tailed P = 0.027 (D, TgGluSensor::OE-Tgmst1); *significant difference to TgGluSensor::CON at two-tailed P = 0.018 (E, TgGluSensor::OE-Tgmst1)