Fig. 7

Function of Tggrp on lignocellulolytic response and transcription of Tgmst1 and Tgmst2. A The growth status of strains (WT, OE-Tggrp, and ∆Tggrp) on MM + straw, MM + glucose, and PDA, grown at 28 °C for 3 days, note that growth of strains was not different in PDA, but there were differences on MM + straw and MM + glucose. B FoldChange of OE-Tggrp relative to WT, 45.41 folds up-regulation in OE-Tggrp. C Hyphae biomass of strains (WT, OE-Tggrp, and ∆Tggrp), grown on MM + straw at 28 °C for 4 days. D FPA of strains (WT, OE-Tggrp, and ∆Tggrp) on MM + straw, grown at 28 °C for 4 days. Overexpression of Tggrp enhanced enzyme activity, while deletion led to a decrease. E The intracellular glucose content of strains (WT, OE-Tggrp, and ∆Tggrp). overexpression of Tgmst1 increased intracellular glucose content. F FoldChange of Tgmst1 in OE-Tggrp and ∆Tggrp. Tgmst1 and Tgmst2 were up-regulated by 369.1 folds and 151.7 folds respectively in OE-Tggrp. G Western blot of MST1 and MST2 in OE-Tggrp and WT, see Additional file 1: Fig. S6D for original image. G1, 2 Western blot of MST1/2 andβ-actin. G3 FoldChange of MST1 and MST2 in OE-Tggrp, MST1 and MST2 were respectively up-regulated 1.7 and 1.8 folds in OE-Tggrp. Bars represent mean ± SEM, with n = 3 biological repeats; red dots resemble values from individual experiments. Student’s t-testing was conducted in (C, D, E), ***significant difference to WT at two-tailed P = 0.00046 (C, OE-Tggrp), **significant difference to WT at two-tailed P = 0.0028 (C, ∆Tggrp); **significant difference to WT at two-tailed P = 0.0012 (D, OE-Tggrp), 0.0086 (D, ∆Tggrp); *significant difference to WT at two-tailed P = 0.011 (E, OE-Tggrp), 0.043 (E, ∆Tggrp)