Fig. 1
Proteomics data overview. A Growth of CC-125 and Mut-5 cultivated under standard conditions to determine time-point selection for comparative proteomics. Data points represent the mean of three independent replicates; error bars indicate the standard deviation from the mean. B Volcano plot showing −Log10-transformed adjusted p-values against the Log2 fold changes for reproducibly quantifiable proteins. Log2 fold change is presented as Mut-5/CC-125. Data points coloured blue and red represent proteins of increased and decreased abundance in Mut-5, respectively. Dotted lines represent the cut-offs for significantly differential protein abundance between Mut-5 and CC-125 (adjusted p-value < 0.05; Log2 fold change < -1 or < 1). C Bar plot showing the top 20 biological process gene ontology (GO) terms enriched in proteins more abundant in Mut-5 compared to CC-125. The number of significantly upregulated proteins associated with each biological process GO term is indicated to the right of each bar. −Log10-transformed false discovery rate is indicated by colour. D Bar plot showing the top 20 biological process gene ontology terms enriched in proteins less abundant in Mut-5 compared to CC-125. The number of significantly downregulated proteins associated with each biological process GO term is indicated to the right of each bar. −Log10-transformed false discovery rate is indicated by colour