Fig. 3
Pigment and qE protein accumulation in CC-125 and Mut-5 cultured in three light conditions. Pigment contents and qE protein accumulation in C. reinhardtii strains CC-125 (blue) and Mut-5 (red) acclimated to low light (LL; 70 µmol photons m2 s−1), medium light (ML; 150 µmol photons m2 s−1), and high light (HL; 400 µmol photons m2 s−1). Total chlorophyll (Chl) content in pg per cell A, total carotenoid (Car) content in pg per cell B, Chl a/Chl b ratios C, and Chl/Car ratios D are displayed. Data are represented as the mean of 3 independent replicates with error bars depicting standard deviation from the mean. For each parameter, significant differences between each strain and light condition were calculated by two-way ANOVA with a multiple comparisons test (compare cell means regardless of rows and columns) and post hoc Bonferroni correction. Means marked with the same letter are not significantly different (p-value > 0.05). E Immunoblots showing LHCSR1, LHCSR3, and PSBS expression in CC-125 (WT) and Mut-5 (M5) under LL, ML, and HL. Antibodies detecting α-ATPase β-subunit were included as a control