Proteomic characterization of a lutein-hyperaccumulating Chlamydomonas reinhardtii mutant reveals photoprotection-related factors as targets for increasing cellular carotenoid content

Table 1 Pigment contents of C. reinhardtii CC-125 and nine high light- and norflurazon-resistant mutant strains

Strain	Chl a (pg cell⁻¹)	Chl b (pg cell⁻¹)	Total Chl (pg cell⁻¹)	Total Car (pg cell⁻¹)	Lutein Content
Strain	Chl a (pg cell⁻¹)	Chl b (pg cell⁻¹)	Total Chl (pg cell⁻¹)	Total Car (pg cell⁻¹)	fg cell⁻¹	mg g⁻¹ DCW
CC-125	1.13 ± 0.26	0.57 ± 0.12	1.70 ± 0.38	0.25 ± 0.05	151 ± 24	1.77 ± 0.18
Mut-1	1.15 ± 0.20	0.69 ± 0.08	1.84 ± 0.28	0.31 ± 0.07	206 ± 25	1.89 ± 0.08
Mut-2	1.84 ± 0.24	1.02 ± 0.11^*	2.86 ± 0.35	0.45 ± 0.04	335 ± 7^*	2.43 ± 0.07
Mut-3	0.76 ± 0.24	0.41 ± 0.15	1.18 ± 0.37	0.20 ± 0.06	133 ± 61	1.90 ± 0.66
Mut-4	1.25 ± 0.24	0.75 ± 0.15	1.99 ± 0.39	0.39 ± 0.06	281 ± 26	2.60 ± 0.12
Mut-5	3.44 ± 0.70^****	1.74 ± 0.25^****	5.18 ± 0.94^****	0.92 ± 0.18^****	806 ± 91^****	4.13 ± 0.4^****
Mut-6	2.67 ± 0.19^**	1.50 ± 0.09^***	4.16 ± 0.28^***	0.65 ± 0.06^**	468 ± 57^***	2.97 ± 0.48^*
Mut-7	2.02 ± 0.58	1.10 ± 0.34^*	3.12 ± 0.92^*	0.51 ± 0.15^*	330 ± 123^*	2.58 ± 0.33
Mut-8	0.86 ± 0.20	0.55 ± 0.18	1.41 ± 0.38	0.29 ± 0.04	190 ± 41	1.98 ± 0.53
Mut-9	0.81 ± 0.07	0.47 ± 0.04	1.28 ± 0.11	0.23 ± 0.03	143 ± 37	2.36 ± 0.32

Isolated strains were scaled up to 25 mL culture volumes and harvested after 4 days. Chlorophyll (Chl) a, Chl b, total Chl and total carotenoid (Car) measurements were estimated using a spectrophotometer [40]. Lutein content was measured by high-performance liquid chromatography against a lutein standard. DCW, dry cell weight. Data represent mean values (n = 3, except for Mut-6 for which n = 2) ± the standard deviation from the mean. Multiple comparisons were performed using one-way ANOVA, with a Bonferroni post hoc test, to compare each mutant to the CC-125 parental strain (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)

ISSN: 2731-3654