Table 1 List of plasmids used in this study
Plasmid name | Relevant genotype | References |
|---|---|---|
pCfB2312 | TEF1p-Cas9-CYC1t; kanMX | [38] |
pCfB2899 | X-2 MarkerFree backbone | [38] |
pCfB3037 | XI-5-MarkerFree backbone | [38] |
pCfB3040 | XII-4- MarkerFree backbone | [38] |
pCfB3020 | gRNA_X-2; natMX | [38] |
pCfB3046 | gRNA_XI-5; natMX | [38] |
pCfB3049 | gRNA_XII-4; natMX | [38] |
pCfB3053 | gRNA_X-2; gRNA_XI-5; gRNA_XII-4; natMX | [38] |
pLWA25 | gRNA_GRE3; natMX | [39] |
pLWA31 | pCfB3037; TDH3p-SpXYL1.2-ADH1t, TEF1p-SsXYL2-GPM1t, PGI1p-XKS1-PYK1t | [21] |
pLWA33 | pCfB3040; TDH3p-SpXYL1.2-ADH1t, TEF1p-SsXYL2-GPM1t, PGI1p-XKS1-PYK1t | [21] |
YEpHXT_PiroXI | pHXT7; HXT7*p-xylA (Piromyces)-PGK1t; URA3 | [14] |
pRS42N | TEF1p-natMX-ADH1t | [40] |
pVP001 | pUC57; PGK1p-xylM-CYC1t | This study |
pUC57 + ClosXI | pUC57; TEF1p- LpXImut2 -GPM1t | This study |
pUC57 + ParaXI | pUC57; xylA (Parabacteroides) | This study |
pRP021 | pCfB2899; TEF1p-LpXImut2-GPM1t; PGI1p-XKS1-PYK1t | This study |
pRP023 | pCfB3037; TEF1p-LpXImut2-GPM1t; PGI1p-XKS1-PYK1t | This study |
pRP024 | pCfB2899; TEF1p-xylA(Parabacteroides)-GPM1t; PGI1p-XKS1-PYK1t | This study |
pRP026 | pCfB3037; TEF1p-xylA(Parabacteroides)-GPM1t; PGI1p-XKS1-PYK1t | This study |
pRP027 | pCfB2899; TEF1p-xylA(Piromyces)-GPM1t; PGI1p-XKS1-PYK1t | This study |
pRP029 | pCfB3037; TEF1p-xylA(Piromyces)-GPM1t; PGI1p-XKS1-PYK1t | This study |
pRP032 | pRS42N; TEF1p-LpXImut2-GPM1t | This study |
pRP033 | pRS42N; TEF1p-xylA (Parabacteroides)-GPM1t | This study |
pRP034 | pRS42N; TEF1p-xylA (Piromyces)-GPM1t | This study |