Fig. 2From: Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impuritiesAnalysis of the recombinant 2eGDHt overexpressed from E. coli BL31 (DE3) by SDS-PAGE and Native-PAGE. A SDS-PAGE analysis. Lane M, molecular weight marker; Lane 1, total cellular proteins expressed from E. coli BL31 (DE3) harboring empty vector induced by IPTG; Lane 2, total cellular proteins expressed from E. coli BL31 (DE3) without inducer; Lane 3, total cellular proteins expressed from E. coli BL31 (DE3) induced by IPTG; Lane 4, purified recombinant 2eGDHt. B Native-PAGE analysis of purified recombinant 2eGDHtBack to article page