Fig. 3

Biochemical characterization of 2eGDHt. A Effect of temperature on the 2eGDHt activity. The enzyme activity was determined at temperatures ranging from 4 to 65 °C at pH 7.0. B Effect of temperature on the 2eGDHt stability. The enzymatic activity was measured at 37 °C and pH 7.0 after preincubation at temperatures ranging from 4 to 65 °C. C Effect of pH on the 2eGDHt activity. The enzymatic activity was determined at pH values ranging from 4.2 to 9.0 at 37 °C. D Effect of temperature on the 2eGDHt stability. The enzymatic activity was measured at 37 °C and pH 7.0 after preincubation at pH values ranging from 4.2 to 9.0. E Effects of various metal ions on the enzymatic activity of 2eGDHt. F Effects of various chemical reagents on the enzymatic activity of 2eGDHt