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Fig. 6 | Biotechnology for Biofuels and Bioproducts

Fig. 6

From: Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impurities

Fig. 6

Comparison of 2eGDHt and mutant I744V. A Root-mean-square fluctuation (RMSF) analysis of 2eGDHt and mutant I744V under different conditions with molecular dynamics (MD) simulations. B Structures of 2eGDHt and mutant I744V colored by normalized RMSF values under different conditions. The thicker coils showed higher flexibility relative to other parts of the protein. The red circle indicates the residue isoleucine at position 744. C Root-mean-square deviation (RMSD) analysis of 2eGDHt and mutant I744V with molecular dynamics (MD) simulations under different conditions. D Effects of mutant I744V on the enzymatic activity of 2eGDHt in pure glycerol and crude glycerol. Asterisks indicate statistically significant differences compared to the WT (***p < 0.001, n = 3)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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