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Table 1 The substrate specificity of 2eGDHt

From: Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impurities

Substrate

Specific activity (U/mg)

Glycerol

0.98 ± 0.05

1,2-Propanediol

0.79 ± 0.03

1,2-Butanediol

0.64 ± 0.05

Butanol

0.53 ± 0.01

Methanol

0.58 ± 0.08

Ethanol

0.52 ± 0.02

Isopropanol

0.51 ± 0.03

  1. The substrate specific activities were determined by incubated with each of the 0.2 M substrates and incubated for 10 min at 37 °C and pH 7.0. Results are presented as means ± standard deviation (n = 3)
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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