Skip to main content

Table 2 Kinetic parameters of 2eGDHt with glycerol and 1,2-PDO

From: Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impurities

Substrate

Km (mM)

Vmax (nkat mg−1)

Kcat (s−1)

Kcat/Km (s−1 mM−1)

Glycerol

3.42

58.15

181.72

53.17

1,2-PDO

3.70

106.65

172.29

46.48

  1. Enzyme assay was performed by incubated with each of the substrates ranging from 2 to 200 mM at 37 °C and pH 7.0 for 10 min
Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us