Biochemical and molecular characterization of a novel glycerol dehydratase from Klebsiella pneumoniae 2e with high tolerance against crude glycerol impurities

Table 3 Effects of various concentrations of main impurities of crude glycerol, including NaCl, methanol, oleic acid, and linoleic acid on the enzymatic activity of 2eGDHt

Impurity	Concentration (%, v/v)	Relative activity (%)
Control	0	100
NaCl	2	102.1 ± 2.8
	4	97.6 ± 2.5
	6	94.1 ± 3.2
	8	91.9 ± 3.4
Methanol	7.5	153.6 ± 2.2
	10	131.4 ± 1.5
	12.5	110.7 ± 2.9
	15	98.8 ± 3.1
Oleic acid	0.6	87.5 ± 3.4
	0.8	84.6 ± 2.8
	1.0	80.4 ± 1.7
	1.2	78.7 ± 2.6
Linoleic acid	0.6	83.4 ± 1.8
	0.8	80.8 ± 2.1
	1.0	78.2 ± 3.2
	1.2	70.3 ± 1.6
Mixture	6% NaCl, 12% methanol, 0.5% oleic acid and 0.5% linoleic acid	97.6 ± 2.3

The activity of 2eGDHt was determined by incubated with the addition of various concentrations of the impurities and the mixture for 10 min at 37 °C and pH 7.0. Enzyme activity without addition of impurity was taken as 100%. Results are represented mean ± SD from triplicate experiments

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