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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production

Fig. 2

Analysis of changes in P. pastoris cell wall permeability after knocking out ΔPAS-0305. Assessment of whole-cell permeability via a intracellular Nile red fluorescence measurement; b intracellular propidium iodide fluorescence measurement; and c measurement of CMCase activity of heterologously HgCel5A. d Comparison of P. pastoris extracellular protein. d1, SDS-PAGE. d2, extracellular protein concentration analysis

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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