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Table 1 The methanol utilization rate and carbon loss parameters calculated using the iMT1026 v3.0 GSMM with the macromolecular composition of cells grown on methanol used as input for defining the stoichiometric coefficients

From: Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production

 

Parameter

P. pastoris X33

ΔPAS_0305

Input

Protein (%)

56.62

67.21

Carbohydrates (%) (β-1,3glucan + chitin + mannose)

27.90 (14.62 + 4.31 + 8.97)

21.09 (16.37 + 1.58 + 3.14)

Lipids (%)

6.50

7.21

Others (%)

8.98

4.51

Output

Methanol utilization rate (mmol/gDCW/h)

1.24

1.45

Carbon loss (mmolCO2/mmol methanol)

1.16

1.06