Table 3 Microbial strains used in the present study
Strains | Relevant genotype | Source of reference |
|---|---|---|
E. coli DH5α | Φ80dlacZΔm15, recA1, endA1, gyrA96, thi-1, hsdR17 (rk−, mk+), supE44, relA1, deoR, Δ(lacZYA-argF) U169 | Invitrogen |
Y. lipolytica Po1f | MatA, leu2-270, ura3-302, xpr2-322 axp1 | Madzak et al., 2000 |
po1f-1 | Po1f; ΔMEF1, ΔPEX10, ΔTGL4 | This investigation |
po1f-control | Po1f; URA3, LEU2 | This investigation |
ylP | Po1f; pTEF-Ctcdp | This investigation |
ylPT | Po1f; pTEF-NcCDT1, pTEF-Ctcdp | This investigation |
ylBPT | Po1f; pTEF-NcCDT1, pTEF-Ctcdp, pTEF-Ctcdp, pTEF-ScPGM2 | This investigation |
yl4BPT | Po1f; p4UTef-NcCDT1, p4UTef-Ctcdp, p4UTef-Ctcdp, pTEF-ScPGM2 | This investigation |
yl8BPT | Po1f; p8UTef-NcCDT1, p8UTef-Ctcdp, p8UTef-Ctcdp, pTEF-ScPGM2 | This investigation |
ylXHK | Po1f; pTEF-SsXR, pTEF-SsXDH, pTEF-XK | This investigation |
yl4XRHK | Po1f; p4UTef-SsXR, p4UTef-SsXDH, p4UTef-XK, pTEF-GiGXF1, pTEF-ZWF1, pTEF-GND1 | This investigation |
yl4BX | yl4BPT; p4UTef-SsXR, p4UTef-SsXDH, p4UTef- XK, pTEF-GiGXF1, pTEF-ZWF1, pTEF-GND1 | This investigation |
yl4BXP | po1f-1; yl4BPT; p4UTef-SsXR, p4UTef-SsXDH, p4UTef- XK, pTEF-GiGXF1, pTEF-ZWF1, pTEF-GND1, pTEF-GPD1, pTEF-DGA2 | This investigation |