Fig. 2

The protein purification of CDW-xyl-8 and CDW-xyl-16. A Lane 1, protein supernatant of E. coli BL21 (DE3) expressed empty plasmid as a negative control; Lane 2, total protein mixture of E. coli BL21 (DE3) expressed CDW-xyl-8; Lane 3, supernatant protein mixture of E.coli BL21 (DE3) expressed CDW-xyl-8; Lane 4, precipitated protein mixture of E. coli BL21 (DE3) expressed CDW-xyl-8; Lane 5, Ni column flow-through solution after protein absorption; Lane 6, protein washed with 20 mM imidazole buffer; Lane 7, protein washed with 50 mM imidazole buffer; Lane 8, protein washed with 100 mM imidazole buffer; Lane 9, protein washed with 300 mM imidazole buffer; Lane M, molecular mass standards. B The same sequence of loading samples for CDW-xyl-16 SDS-PAGE results