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Table 3 qRT-PCR of differentially expressed genes following N deprivation

From: Lipid accumulation mechanism of Amphora coffeaeformis under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture

Gene

Location

Forward (5′–3′)

Reverse (5′–3′)

Log2FC (qRT-PCR)

Log2FC (RNA-seq)

gpat

Cluster-30447.20498

ACCTTGAAGGTT

ACGGTATTGCTC

1.248

1.241

  

TCCATGGCCTT

GGGCATACTTT

  

lpaat

Cluster-30447.18970

TTTGTTGCCTCG

TTGCAGGATACC

 − 1.475

 − 1.184

  

GCCTGGCA

AAACATGACGG

  

pdat

Cluster-30447.20296

TTATCGCTCCTTC

GCATCCACAGG

 − 1.222

 − 1.768

  

CATTCTTTCG

AGAAACCATC

  
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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