Fig. 6

Effect of target gene mutation on expression of α-amylase in A. oryzae. a Schematic diagram of target gene editing based on the CRISPR–Cas9 system. b α-Amylase activity of different mutants related to the target genes. c Effect of mutations in different regions of AO090026000500 and AO090001000601 on the expression of α-amylase in A. oryzae. Strain SpX-N indicates that the mutation occurs near Spacer X, and N indicates the number of different mutant strains