Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization

Table 1 Central composite design matrix of optimization variables with actual values and response variable for ethanol production

Run	Hydrolysate concentration (%)	Temperature ºC	Time H	Ethanol concentration (%)
Run	Hydrolysate concentration (%)	Temperature ºC	Time H	Actual value	Predicted value
(a) Escherichia coli K12
1	1.47759	33.5	5.5	0.0023	0.0026
2	22.5	33.5	5.5	0.0020	0.0020
3	35	30	8	0.0042	0.0040
4	10	37	3	0.0018	0.0016
5	22.5	33.5	5.5	0.0020	0.0020
6	22.5	33.5	5.5	0.0020	0.0020
7	22.5	33.5	1.29552	0.0020	0.0023
8	22.5	33.5	9.70448	0.0019	0.0022
9	10	37	8	0.0021	0.0019
10	22.5	27.6137	5.5	0.0023	0.0025
11	43.5224	33.5	5.5	0.0025	0.0028
12	10	30	3	0.0035	0.0033
13	35	37	8	0.0023	0.0021
14	35	30	3	0.0024	0.0022
15	22.5	39.3863	5.5	0.0025	0.0028
16	10	30	8	0.0027	0.0025
17	22.5	33.5	5.5	0.0020	0.0020
18	22.5	33.5	5.5	0.0020	0.0020
19	22.5	33.5	5.5	0.0020	0.0020
20	35	37	3	0.0027	0.0025
(b) Saccharomyces cerevisiae S288c (ATCC 7754)
1	10	30	8	0.0181	0.0163
2	22.5	35	5.5	0.0056	0.0056
3	35	40	8	0.0022	0.0027
4	22.5	35	9.7	0.0071	0.0071
5	22.5	35	1.29	0.0046	0.0043
6	22.5	35	5.5	0.0056	0.0056
7	22.5	43.409	5.5	0.0054	0.0033
8	10	40	3	0.0064	0.0065
9	35	30	8	0.0072	0.0073
10	43.5224	35	5.5	0.0051	0.0043
11	10	30	3	0.0119	0.0116
12	22.5	35	5.5	0.0056	0.0056
13	22.5	26.591	5.5	0.0097	0.0115
14	22.5	35	5.5	0.0056	0.0056
15	22.5	35	5.5	0.0056	0.0056
16	1.47759	35	5.5	0.0135	0.014
17	35	40	3	0.002	0.004
18	10	40	8	0.0059	0.0072
19	22.5	35	5.5	0.0056	0.0056
20	35	30	3	0.0058	0.0047

ISSN: 2731-3654