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Table 6 Experimental range and levels used in central composite design matrix for ethanol production

From: Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization

Independent variables

Range and level

Symbol

− α

− 1

0

1

 + α

(a) Escherichia coli K12

 Hydrolysate concentration (%)

X1

1.48

10.00

22.5

35.00

43.52

 Temperature (oC)

X2

27.61

30.00

33.50

37.00

39.39

 Time (H)

X3

1.30

3.00

5.50

8.00

9.07

(b) Saccharomyces cerevisiae S288c (ATCC 7754)

 Hydrolysate concentration (%)

X1

1.48

10.00

22.5

35.00

43.52

 Temperature (oC)

X2

26.59

30.00

35.0

40.00

43.41

 Time (H)

X3

1.30

3.00

5.50

8.00

9.07