Metabolic engineering of a stable haploid strain derived from lignocellulosic inhibitor tolerant Saccharomyces cerevisiae natural isolate YB-2625

Table 1 Furfural and HMF remaining at 96 h

Strain	Parent	Furfural [g/L]	HMF [g/L]
YRH2066	YRH1946	0.00 ± 0.001	0.01 ± 0.002
YRH2073	BY4741	0.32 ± 0.095	0.33 ± 0.019
YRH2074	CEN.PK2-1C	0.00 ± 0.000	0.13 ± 0.043

Initial furfural and HMF concentrations present in the medium were 1.21 g/L and 0.44 g/L, respectively. Each parent strain listed above was engineered in the same manner to replace PHO13 with the genes required for xylose fermentation (i.e., pho13∆::P_PGK1-XYL1-T_PGK1; P_ADH1-XYL2-T_ADH1; P_HXT7-XKS1-T_HXT7). Data shown are the mean values determined from at least three biological replicates ± standard deviations

ISSN: 2731-3654